Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Mar-Apr;15(2):020506.
doi: 10.1117/1.3369966.

Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells

YongKeun Park  1 , Monica Diez-Silva, Dan Fu, Gabriel Popescu, Wonshik Choi, Ishan Barman, Subra Suresh, Michael S Feld
Affiliations

Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells

YongKeun Park et al. J Biomed Opt. 2010 Mar-Apr.

Abstract

We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.

PubMed Disclaimer

Figures

Figure 1
Figure 1
(a) Amplitude and (b) phase map of a healthy RBC. (c) The retrieved light scattering pattern of the same cell. Light-intensity scattering patterns of (d) healthy RBCs, (e) ring, (f) trophozoite, and (g) schizont stage of Pf-RBCs. (h) p-values of scattering patterns (different intraerythrocytic stages of Pf-RBCs are compared to the healthy RBCs).
Figure 2
Figure 2
Dynamic light scattering of Pf-RBCs. (a) Normalized temporal autocorrelations of a healthy RBC as a function of decay time and scattering angle. (b) Normalized temporal autocorrelation of a healthy RBC at two representative scattering angles and fitted curves. (c) Line width Γ extracted from healthy and Pf RBCs as a function of scattering angle. Symbols represent the mean value and the error bars indicate the standard error for 15 RBCs. (d) Scatter plot of ω0 versus Γ taken for healthy and Pf RBCs. Each symbol represents mean values of 15 RBCs at scattering angles (0 to 21 deg). Lines indicate the boundaries of each group.
See this image and copyright information in PMC

References

    1. Gurjar R., Backman V., Perelman L., Georgakoudi I., Badizadegan K., Itzkan I., Dasari R., and Feld M., “Imaging human epithelial properties with polarized light-scattering spectroscopy,” Nat. Med. ZZZZZZ 7(11), 1245–1248 (2001).10.1038/nm1101-1245 - DOI - PubMed
    1. Berne B. and Pecora R., Dynamic Light Scattering: with Applications to Chemistry, Biology, and Physics, Dover Publications, Mineola, New York: (2000).
    1. Pine D., Weitz D., Chaikin P., and Herbolzheimer E., “Diffusing wave spectroscopy,” Phys. Rev. Lett. PRLTAO 60(12), 1134–1137 (1988).10.1103/PhysRevLett.60.1134 - DOI - PubMed
    1. Amin M., Park Y., Lue N., Dasari R., Badizadegan K., Feld M., and Popescu G., “Microrheology of red blood cell membranes using dynamic scattering microscopy,” Opt. Express OPEXFF 15(25), 17001–17009 (2007).10.1364/OE.15.017001 - DOI - PubMed
    1. Subramanian H., Pradhan P., Liu Y., Capoglu I., Li X., Rogers J., Heifetz A., Kunte D., Roy H., Taflove A., and Backman V., “Optical methodology for detecting histologically unapparent nanoscale consequences of genetic alterations in biological cells,” Proc. Natl. Acad. Sci. U.S.A. PNASA6 105(51), 20118 (2008).10.1073/pnas.0804723105 - DOI - PMC - PubMed

Publication types