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. 2010 Jul;48(7):2569-70.
doi: 10.1128/JCM.02449-09. Epub 2010 May 12.

Comparison of single-copy and multicopy real-time PCR targets for detection of Mycobacterium tuberculosis in paraffin-embedded tissue

Robert F Luo  1 , Michael D Scahill, Niaz Banaei
Affiliations

Comparison of single-copy and multicopy real-time PCR targets for detection of Mycobacterium tuberculosis in paraffin-embedded tissue

Robert F Luo et al. J Clin Microbiol. 2010 Jul.

Abstract

Real-time PCR can rapidly identify Mycobacterium tuberculosis in paraffin-embedded tissue in the absence of microbiological culture. In a comparison of single-copy and multicopy PCR targets in 70 tissue samples, the sensitivities were 26% and 54%, respectively, with 100% specificity. Sensitivity was 75% for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.

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