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Comment
. 2010 May 14;86(5):819-22; author reply 822-3.
doi: 10.1016/j.ajhg.2010.03.019.

Tuba8 is expressed at low levels in the developing mouse and human brain

Andreas Braun, Martin Breuss, Marion C Salzer, Jonathan Flint, Nicholas J Cowan, David A Keays
Comment

Tuba8 is expressed at low levels in the developing mouse and human brain

Andreas Braun et al. Am J Hum Genet. .
No abstract available

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Figures

Figure 1
Figure 1
Tuba8 Expression in the Developing Mouse Brain Coronal sections (14 μm) of the developing cortex (E14.5, E16.5, P0) showing our in situ hybridization results obtained with the probe employed by Sheridan and colleagues (A, D, G) and the short (B, E, H) and long (C, F, I) probe targeted to the unique 3′ UTR of Tuba8. High levels of staining are observed in the developing mouse brain with the Sheridan probe (particularly in the cortical plate [CP] and to a lesser extent in the intermediate zone [IZ] and ventricular zone [VZ]), but none is apparent with probes that exclusively target the 3′ UTR. We observed no staining when employing control sense probes (data not shown). Scale bars represent 50 μm. MZ indicates the marginal zone.
Figure 2
Figure 2
Real-Time qPCR Analysis of Tuba8 Expression Levels in Mouse and Human Brain (A) Relative expression levels of Tuba1a, Tubb2b, Tubb3, and Tuba8 in the developing mouse brain (E10.5 [head], E12.5, E14.5, E16.5, E18.5, P0, and P6). Tuba8 is expressed at very low levels at all developmental stages. (B) In the adult mouse brain, there are regional expression differences when comparing Tuba1a, Tubb2b, Tubb3, and Tuba8. The expression of Tuba8 is again low, with the exception of the olfactory bulbs and cerebellum, where it is expressed at modest levels. (C) Expression of TUBA1A, TUBB2B, TUBB3, and TUBA8 in the developing human brain at GW13 (frontal lobe) and GW22 (total fetal brain). Again, TUBA8 is expressed at low levels. Error bars indicate the SEM.
See this image and copyright information in PMC

Comment on

References

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