Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Aug;26(8):779-85.
doi: 10.1016/j.dental.2010.04.002. Epub 2010 May 15.

Substantivity of chlorhexidine to human dentin

Marcela R Carrilho  1 , Ricardo M Carvalho, Ethan N Sousa, José Nicolau, Lorenzo Breschi, Annalisa Mazzoni, Leo Tjäderhane, Franklin R Tay, Kelli Agee, David H Pashley
Affiliations

Substantivity of chlorhexidine to human dentin

Marcela R Carrilho et al. Dent Mater. 2010 Aug.

Abstract

Objectives: To better comprehend the role of CHX in the preservation of resin-dentin bonds, this study investigated the substantivity of CHX to human dentin.

Material and methods: Dentin disks (n=45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: (1) 10 microL of distilled water (controls), (2) 10 microL of 0.2% chlorhexidine diacetate (0.2% CHX) or (3) 10 microL of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 degrees C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5 h, 1 h, 3 h, 6 h, 24 h, 168 h (1 week), 672 h (4 weeks) and 1344 h (8 weeks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm.

Results: Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation (p<0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) (p<0.05).

Conclusion: The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin-dentin bonds.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Chemical structure of chlorhexidine.
Figure 2
Figure 2
Percentage of bound CHX to specimens treated with 0.2% (A) CHX or treated with 2% CHX (B) as a function of time. The percentage bound is relative to the amount applied. That is, little CHX remained bound to hydroxyapatite (HA), while most of the CHX applied to the various dentins remained bound.
Figure 2
Figure 2
Percentage of bound CHX to specimens treated with 0.2% (A) CHX or treated with 2% CHX (B) as a function of time. The percentage bound is relative to the amount applied. That is, little CHX remained bound to hydroxyapatite (HA), while most of the CHX applied to the various dentins remained bound.
See this image and copyright information in PMC

References

    1. Nerurkar MJ, Zentner GM, Howard Rytting J. Effect of chloride in the releasing of chlorhexidine salts from methyl methacrylate: 2-hydroxyethyl methacrylate copolymer reservoir devices. J Control Release. 1995;33:357–363.
    1. Siqueira JF, Jr, Paiva SS, Rôças IN. Reduction in the cultivable bacterial populations in infected root canals by a chlorhexidine-based antimicrobial protocol. J Endod. 2007;33:541–547. - PubMed
    1. Vianna ME, Horz HP, Conrads G, Feres M, Gomes BP. Comparative analysis of endodontic pathogens using checkerboard hybridization in relation to culture. Oral Microbiol Immunol. 2008;23:282–290. - PubMed
    1. Cosyn J, Wyn I, De Rouck T, Sabzevar MM. Long-term clinical effects of a chlorhexidine varnish implemented treatment strategy for chronic periodontitis. J Periodontol. 2006;77:406–415. - PubMed
    1. Duarte FF, Lotufo RF, Pannuti CM. Local delivery of chlorhexidine gluconate in patients with aggressive periodontitis. J Int Acad Periodontol. 2008;10:31–35. - PubMed

Publication types

MeSH terms