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. 1991 Jan;163(1):31-7.
doi: 10.1620/tjem.163.31.

Detection of methicillin-resistant Staphylococcus aureus (MRSA) using polymerase chain reaction amplification

Y Tokue  1 , S Shoji, K Satoh, A Watanabe, M Motomiya
Affiliations
Free article

Detection of methicillin-resistant Staphylococcus aureus (MRSA) using polymerase chain reaction amplification

Y Tokue et al. Tohoku J Exp Med. 1991 Jan.
Free article

Abstract

Polymerase chain reaction (PCR) was applied for the detection of methicillin-resistant Staphylococcus aureus (MRSA). This procedure amplified a segment of MRSA-PBP (penicillin-binding protein) gene of DNA extract from the clinical isolates of S. aureus. A 1339-base-pair fragment of MRSA-PBP gene in DNA from S. aureus (MIC of methicillin, greater than or equal to 16 micrograms/ml) was amplified and detected by a specific oligonucleotide probe. Moreover, a 4.3 kb HindIII fragment containing MRSA-PBP gene was detected by using the same oligonucleotide probe. On the other hand, no PCR-amplified product was detected in DNA from methicillin-sensitive S. aureus (MIC of methicillin, less than 16 micrograms/ml).

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