Cytotoxic effects of orthodontic composites

Abstract

Objectives: To evaluate the cytotoxic effects of five different light-cured orthodontic bonding composites.

Materials and methods: The orthodontic composites Heliosit Orthodontic (Ivoclar), Transbond XT (3M Unitek), Bisco ORTHO (Bisco), Light Bond (Reliance), and Quick Cure (Reliance) were prepared, and the samples were extracted in 3 mL of BME (Basal Medium Eagle) with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in a 96-well dish and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24 hours of incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then, L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyl tetrazolium (MTT) test. Twelve wells were used for each specimen, and the MTT tests were applied two times. The data were statistically analyzed by one-way analysis of variance (ANOVA) and Tukey HSD tests.

Results: Results with L929 fibroblasts demonstrated that except for Transbond XT, freshly prepared composite materials did not reduce vital cell numbers (P > .05) compared with the control group. Our data demonstrate that Transbond XT showed significant cytotoxicity compared with the control group.

Conclusion: Results indicate that tested orthodontic bonding composites are suitable for clinical application, but that further studies using different test methods are needed for Transbond XT.

Figure 1

Cultured L929 cells for control group.

Figure 2

Cultured L929 cells for Heliosit Orthodontic.

Figure 3

Cultured L929 cells for Transbond XT.

Figure 4

Cultured L929 cells for Bisco ORTHO.

Figure 5

Cultured L929 cells for Light Bond.

Figure 6

Cultured L929 cells for Quick Cure.