Timing of CD8+ T cell responses in relation to commencement of capillary leakage in children with dengue
- PMID: 20483770
- PMCID: PMC4340505
- DOI: 10.4049/jimmunol.0903262
Timing of CD8+ T cell responses in relation to commencement of capillary leakage in children with dengue
Abstract
Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38+/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from children with dengue, we found no evidence of increased CD8+ T cell activation prior to the commencement of resolution of viremia or hemoconcentration. Investigations with MHC class I tetramers to detect NS3(133-142)-specific CD8+ T cells in two independent cohorts of children suggested the commencement of hemoconcentration and thrombocytopenia in DHF patients generally begins before the appearance of measurable frequencies of NS3(133-142)-specific CD8+ T cells. The temporal mismatch between the appearance of measurable surface activated or NS3(133-142)-specific CD8+ T cells suggests that these cells are sequestered at sites of infection, have phenotypes not detected by our approach, or that other mechanisms independent of CD8+ T cells are responsible for early triggering of capillary leakage in children with DHF.
Figures





Similar articles
-
Dominant recognition by human CD8+ cytotoxic T lymphocytes of dengue virus nonstructural proteins NS3 and NS1.2a.J Clin Invest. 1996 Oct 1;98(7):1684-91. doi: 10.1172/JCI118964. J Clin Invest. 1996. PMID: 8833919 Free PMC article.
-
Characterization of Human CD8 T Cell Responses in Dengue Virus-Infected Patients from India.J Virol. 2016 Nov 28;90(24):11259-11278. doi: 10.1128/JVI.01424-16. Print 2016 Dec 15. J Virol. 2016. PMID: 27707928 Free PMC article.
-
T cell responses to an HLA-B*07-restricted epitope on the dengue NS3 protein correlate with disease severity.J Immunol. 2002 Jun 1;168(11):5959-65. doi: 10.4049/jimmunol.168.11.5959. J Immunol. 2002. PMID: 12023403
-
Differential targeting of viral components by CD4+ versus CD8+ T lymphocytes in dengue virus infection.J Virol. 2013 Mar;87(5):2693-706. doi: 10.1128/JVI.02675-12. Epub 2012 Dec 19. J Virol. 2013. PMID: 23255803 Free PMC article.
-
Understanding the contribution of cellular immunity to dengue disease pathogenesis.Immunol Rev. 2008 Oct;225:300-13. doi: 10.1111/j.1600-065X.2008.00678.x. Immunol Rev. 2008. PMID: 18837790 Review.
Cited by
-
Peripheral Follicular T Helper Cells and Mucosal-Associated Invariant T Cells Represent Activated Phenotypes During the Febrile Phase of Acute Dengue Virus Infection.Viral Immunol. 2020 Nov;33(9):610-615. doi: 10.1089/vim.2020.0149. Epub 2020 Sep 30. Viral Immunol. 2020. PMID: 32996843 Free PMC article.
-
CD8+ T lymphocyte expansion, proliferation and activation in dengue fever.PLoS Negl Trop Dis. 2015 Feb 12;9(2):e0003520. doi: 10.1371/journal.pntd.0003520. eCollection 2015 Feb. PLoS Negl Trop Dis. 2015. PMID: 25675375 Free PMC article.
-
Evolution of the innate and adaptive immune response in women with acute Zika virus infection.Nat Microbiol. 2020 Jan;5(1):76-83. doi: 10.1038/s41564-019-0618-z. Epub 2019 Dec 2. Nat Microbiol. 2020. PMID: 31792427 Free PMC article.
-
T-cell immunity to infection with dengue virus in humans.Front Immunol. 2014 Mar 7;5:93. doi: 10.3389/fimmu.2014.00093. eCollection 2014. Front Immunol. 2014. PMID: 24639680 Free PMC article. Review.
-
Elucidating the role of T cells in protection against and pathogenesis of dengue virus infections.Future Microbiol. 2014;9(3):411-25. doi: 10.2217/fmb.13.171. Future Microbiol. 2014. PMID: 24762312 Free PMC article. Review.
References
-
- Srikiatkhachorn A, Krautrachue A, Ratanaprakarn W, Wongtapradit L, Nithipanya N, Kalayanarooj S, Nisalak A, Thomas SJ, Gibbons RV, Mammen MP, Jr., et al. Natural history of plasma leakage in dengue hemorrhagic fever: a serial ultrasonographic study. Pediatr. Infect. Dis. J. 2007;26:283–290. discussion 291-292. - PubMed
-
- Gamble J, Bethell D, Day NP, Loc PP, Phu NH, Gartside IB, Farrar JF, White NJ. Age-related changes in microvascular permeability: a significant factor in the susceptibility of children to shock? Clin. Sci. (Lond.) 2000;98:211–216. - PubMed
-
- Loke H, Bethell D, Phuong CX, Day N, White N, Farrar J, Hill A. Susceptibility to dengue hemorrhagic fever in Vietnam: evidence of an association with variation in the vitamin d receptor and Fc gamma receptor IIa genes. Am. J. Trop. Med. Hyg. 2002;67:102–106. - PubMed
-
- Loke H, Bethell DB, Phuong CX, Dung M, Schneider J, White NJ, Day NP, Farrar J, Hill AV. Strong HLA class I–restricted T cell responses in dengue hemorrhagic fever: a double-edged sword? J. Infect. Dis. 2001;184:1369–1373. - PubMed
-
- Graham RR, Juffrie M, Tan R, Hayes CG, Laksono I, Ma’roef C, Erlin, Sutary, Porter KR, Halstead SB. A prospective seroepidemiologic study on dengue in children four to nine years of age in Yogyakarta, Indonesia I. studies in 1995-1996. Am. J. Trop. Med. Hyg. 1999;61:412–419. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials