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. 2010 Mar-Apr;18(2):166-70.
doi: 10.1590/s1678-77572010000200011.

Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries

Luiza Foltran Azevedo  1 , Giovana Daniela Pecharki, João Armando Brancher, Carlos Alberto Cordeiro Jr, Kamilla Gabriella dos Santos Medeiros, Alessandra Armstrong Antunes, Eduardo Silva Arruda, Renata Iani Werneck, Luciana Reis de Azevedo, Rui Fernando Mazur, Samuel Jorge Moysés, Simone Tetü Moysés, Fábio Rueda Faucz, Paula Cristina Trevilatto
Affiliations

Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries

Luiza Foltran Azevedo et al. J Appl Oral Sci. 2010 Mar-Apr.

Abstract

Objective: The present study evaluated the association between lactotransferrin (LTF) gene polymorphism (exon 2, A/G, Lys/Arg) and dental caries.

Material and methods: A convenience sample of 110 individuals, 12 years old, was divided into: group 1, 48 individuals without caries experience (DMFT=0), and group 2, 62 subjects with caries experience (DMFT>or=1). DNA was obtained from a mouthwash with 3% glucose solution, followed by a scrapping of the oral mucosa. After DNA purification, polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) was performed to access the study polymorphism. The LTF A/G (Lys/Arg) polymorphism had been previously reported as located in exon 1.

Results: Allele 1 of the study polymorphism was associated with low DMFT index and showed a protective effect against caries experience (OR=0.16, IC=0.03-0.76, p=0.01).

Conclusions: Lactotransferrin A/G (exon 2, Lys/Arg) polymorphism was associated with susceptibility to dental caries in 12-year-old students.

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Figures

Figure 1
Figure 1
Lactotransferrin (LTF) gene sequence presenting the polymorphism A/G indicated by the letter R. The capital letters refer to exon 2 (GenBank, NM_002343). Underlined boldface bases represent primers that flank the amplified sequence
See this image and copyright information in PMC

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