Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010;24(3):139-44.
doi: 10.1002/jcla.20396.

Evaluation of the alkaline wash/lysis procedure for the molecular diagnosis of a positive bacterial blood culture in clinical routine practice

Sheng-Chuan Hsi  1 , Jun-Ren Sun, Tzong-Shi Chiueh
Affiliations

Evaluation of the alkaline wash/lysis procedure for the molecular diagnosis of a positive bacterial blood culture in clinical routine practice

Sheng-Chuan Hsi et al. J Clin Lab Anal. 2010.

Abstract

Blood culture is commonly used to detect microorganisms in patients with a suspected blood infection. This study evaluated the alkaline wash/lysis procedure to extract DNA of microorganisms in a clinical blood culture. A multiplex polymerase chain reaction (PCR) targeting the 16S rDNA (ribosomal DNA) gene and the fungal ITS (internal transcribed spacer) gene was used as a reliable indicator for the presence of microorganism DNA in the extracts. A total of 535 BacT/ALERT positive blood culture bottles were evaluated. Multiplex PCR showed positive results in 530 DNA extracts, but 5 DNA extracts gave negative results. We conclude that the alkaline wash/lysis procedure in combination with the multiplex PCR is a simple and sensitive method, which can be used in a standard diagnostic laboratory to detect microorganisms in blood culture material.

PubMed Disclaimer

References

    1. Sogaard M, Norgaard M, Schonheyder HC. First notification of positive blood cultures and the high accuracy of the gram stain report. J Clin Microbiol 2007;45:1113–1117. - PMC - PubMed
    1. Grohs P, Mainardi JL, Podglajen I, et al. Relevance of routine use of the anaerobic blood culture bottle. J Clin Microbiol 2007;45:2711–2715. - PMC - PubMed
    1. Karunakaran R, Raja NS, Quek KF, Hoe VC, Navaratnam P. Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system. J Microbiol Immunol Infect 2007;40:445–449. - PubMed
    1. Bouza E, Sousa D, Munoz P, Rodriguez‐Creixems M, Fron C, Lechuz JG. Bloodstream infections: A trial of the impact of different methods of reporting positive blood culture results. Clin Infect Dis 2004;39:1161–1169. - PubMed
    1. Smith JA, Bryce EA, Ngui‐Yen JH, Roberts FJ. Comparison of BACTEC 9240 and BacT/Alert blood culture systems in an adult hospital. J Clin Microbiol 1995;33:1905–1908. - PMC - PubMed

Publication types