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Clinical Trial
. 2010 Jul;15(7):806-10.
doi: 10.1111/j.1365-3156.2010.02548.x. Epub 2010 May 14.

Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

Frank L Basiye  1 , Margaret Mbuchi, Charles Magiri, George Kirigi, Stijn Deborggraeve, Gerard J Schoone, Alfarazdeg A Saad, Sayda El-Safi, Enock Matovu, Monique K Wasunna
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Free article
Clinical Trial

Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

Frank L Basiye et al. Trop Med Int Health. 2010 Jul.
Free article

Abstract

Objective: To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya.

Methods: Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays.

Results: The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively.

Conclusion: Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease.

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