Localization of smooth-muscle markers in the ovaries of some ectothermic vertebrates

Abstract

In the present study, we have localized desmin and alpha-smooth-muscle actin in the ovaries of the zebrafish, the axolotl, and the red-eared turtle, using the unlabelled antibody peroxidase-antiperoxidase technique. In the axolotl ovary both smooth-muscle markers were demonstrated in cord-like structures, extended along ovarian blood vessels, and in some inner ovarian epithelium cells. In the ovaries of the teleost, smooth-muscle-like cells are detected in a suspensory apparatus formed by venous cords, the tunica albuginea, and the coat around the ovarian artery. Also, in the turtle ovary, smooth-muscle-like cells were found in a suspensory apparatus formed by chordae, the tunica albuginea, and the theca externa of the ovarian follicles. At the present time, the prevailing hypothesis is that, in addition to a role in the mechanical support, the smooth-muscle-like cells in the ovaries of these vertebrates seem to be important with respect to ovarian contractile processes.