Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Feb;5(2):615-22.
doi: 10.1093/nar/5.2.615.

Base-specific reactions useful for DNA sequencing: methylene blue--sensitized photooxidation of guanine and osmium tetraoxide modification of thymine

T Friedmann, D M Brown
Free PMC article

Base-specific reactions useful for DNA sequencing: methylene blue--sensitized photooxidation of guanine and osmium tetraoxide modification of thymine

T Friedmann et al. Nucleic Acids Res. 1978 Feb.
Free PMC article

Abstract

Exposure of DNA to methylene blue and visible or ultraviolet light causes guanine-specific modification, and subsequent treatment with piperidine leads to chain cleavage at each guanine residue. Treatment of DNA with osmium tetraoxide in dilute pyridine leads to thymidine-specific modification, and subsequent treatment with piperidine leads to chain cleavage at the modified thymidine residues. Both reactions can be used in conjunction with other base specific modifications described by Maxam and Gilbert (1) for the determination of the nucleotide sequence in DNA.

PubMed Disclaimer

References

    1. J Mol Biol. 1965 May;12:50-9 - PubMed
    1. Arch Biochem Biophys. 1964 Apr;105:197-206 - PubMed
    1. J Mol Biol. 1962 Jun;4:488-99 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3594-7 - PubMed