Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Feb;75(2):645-9.
doi: 10.1073/pnas.75.2.645.

An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion

K Geider, E Beck, H Schaller

An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion

K Geider et al. Proc Natl Acad Sci U S A. 1978 Feb.

Abstract

Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to synthesize an RNA at the origin of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Biol Chem. 1977 Sep 25;252(18):6478-84 - PubMed
    1. Eur J Biochem. 1976 Dec 11;71(2):443-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jan;75(1):50-3 - PubMed
    1. Curr Top Microbiol Immunol. 1976;74:55-112 - PubMed
    1. J Biol Chem. 1974 Jul 10;249(13):3999-4005 - PubMed

MeSH terms