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. 1978 Feb;75(2):645-9.
doi: 10.1073/pnas.75.2.645.

An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion

K Geider, E Beck, H Schaller

An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion

K Geider et al. Proc Natl Acad Sci U S A. 1978 Feb.

Abstract

Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to synthesize an RNA at the origin of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.

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References

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