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. 2010 Jun;26(3):205-10.
doi: 10.1007/s12264-010-1117-7.

Dose- and time-dependent alpha-synuclein aggregation induced by ferric iron in SK-N-SH cells

Wen-Jing Li  1 , Hong Jiang, Ning Song, Jun-Xia Xie
Affiliations

Dose- and time-dependent alpha-synuclein aggregation induced by ferric iron in SK-N-SH cells

Wen-Jing Li et al. Neurosci Bull. 2010 Jun.

Abstract
in English, Chinese

Objective: Intracellular formation of Lewy body (LB) is one of the hallmarks of Parkinson's disease. The main component of LB is aggregated alpha-synuclein, present in the substantia nigra where iron accumulation also occurs. The present study aimed to study the relationship between iron and alpha-synuclein aggregation.

Methods: SK-N-SH cells were treated with different concentrations of ferric iron for 24 h or 48 h. MTT assay was conducted to determine the cell viability. Thioflavine S staining was used to detect alpha-synuclein aggregation.

Results: With the increase of iron concentration, the cell viability decreased significantly. At the concentrations of 5 mmol/L and 10 mmol/L, iron induced alpha-synuclein aggregation more severely than at the concentration of 1 mmol/L. Besides, 48-h treatment-induced aggregation was more severe than that induced by 24-h treatment, at the corresponding iron concentrations.

Conclusion: Ferric iron can induce alpha-synuclein aggregation, which is toxic to the cells, in a dose- and time-dependent way.

目的: 帕金森氏病的一个重要标志是胞浆内路易小体的形成。 路易小体的主要成分是α- 突触核蛋白的聚集体, 它和铁的积聚一同存在于黒质区。 本实验旨在研究铁和α- 突触核蛋白聚集的关系。

方法: SK-N-SH 细胞在不同浓度的三价铁作用下, 分别孵育24 h 或48 h, 用MTT 法和硫磺素S 染色法分别检测细胞存活率、 观察α- 突触核蛋白的聚集情况。

结果: 随着铁离子浓度的升高, 细胞存活率显著下降。 其中5 mmol/L 和10 mmol/L 三价铁引起的α- 突触核蛋白的聚集相较于1 mmol/L 的三价铁引起的聚集更为严重。 此外, 在同一浓度的三价铁作用下, 孵育48 h引起的聚集比孵育24 h引起的聚集更严重。

结论: 铁离子诱发的α-突触核蛋白的聚集具有剂量和时间的依赖性, 并且α- 突触核蛋白的聚集介导了铁的毒性作用。

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References

    1. Halliday G.M., Del Tredici K., Braak H. Critical appraisal of brain pathology staging related to presymptomatic and symptomatic cases of sporadic Parkinson’s disease. J Neural Transm Suppl. 2006;70:99–103. doi: 10.1007/978-3-211-45295-0_16. - DOI - PubMed
    1. Shults C.W. Lewy bodies. Proc Natl Acad Sci U S A. 2006;103:1661–1668. doi: 10.1073/pnas.0509567103. - DOI - PMC - PubMed
    1. Spillantini M.G., Schmidt M.L., Lee V.M., Trojanowski J.Q., Jakes R., Goedert M. α-Synuclein in Lewy bodies. Nature. 1997;388:839–840. doi: 10.1038/42166. - DOI - PubMed
    1. Iwai A., Masliah E., Yoshimoto M., Ge N., Flanagan L., de Silva H.A., et al. The precursor protein of non-Aβ component of Alzheimer’s disease amyloid is a presynaptic protein of the central nervous system. Neuron. 1995;14:467–475. doi: 10.1016/0896-6273(95)90302-X. - DOI - PubMed
    1. Wislet-Gendebien S., D’souza C., Kawarai T., St George-Hyslop P., Westaway D., Fraser P., et al. Cytosolic proteins regulate α-synuclein dissociation from presynaptic membranes. J Biol Chem. 2006;281:32148–32155. doi: 10.1074/jbc.M605965200. - DOI - PubMed

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