Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Multicenter Study
. 2010;14(3):R96.
doi: 10.1186/cc9031. Epub 2010 May 26.

Early alterations of the innate and adaptive immune statuses in sepsis according to the type of underlying infection

Charalambos Gogos  1 , Antigone Kotsaki, Aimilia Pelekanou, George Giannikopoulos, Ilia Vaki, Panagiota Maravitsa, Stephanos Adamis, Zoi Alexiou, George Andrianopoulos, Anastasia Antonopoulou, Sofia Athanassia, Fotini Baziaka, Aikaterini Charalambous, Sofia Christodoulou, Ioanna Dimopoulou, Ioannis Floros, Efthymia Giannitsioti, Panagiotis Gkanas, Aikaterini Ioakeimidou, Kyriaki Kanellakopoulou, Niki Karabela, Vassiliki Karagianni, Ioannis Katsarolis, Georgia Kontopithari, Petros Kopterides, Ioannis Koutelidakis, Pantelis Koutoukas, Hariklia Kranidioti, Michalis Lignos, Konstantinos Louis, Korina Lymberopoulou, Efstratios Mainas, Androniki Marioli, Charalambos Massouras, Irini Mavrou, Margarita Mpalla, Martha Michalia, Heleni Mylona, Vassilios Mytas, Ilias Papanikolaou, Konstantinos Papanikolaou, Maria Patrani, Ioannis Perdios, Diamantis Plachouras, Aikaterini Pistiki, Konstantinos Protopapas, Kalliopi Rigaki, Vissaria Sakka, Monika Sartzi, Vassilios Skouras, Maria Souli, Aikaterini Spyridaki, Ioannis Strouvalis, Thomas Tsaganos, George Zografos, Konstantinos Mandragos, Phylis Klouva-Molyvdas, Nina Maggina, Helen Giamarellou, Apostolos Armaganidis, Evangelos J Giamarellos-Bourboulis
Affiliations
Multicenter Study

Early alterations of the innate and adaptive immune statuses in sepsis according to the type of underlying infection

Charalambos Gogos et al. Crit Care. 2010.

Abstract

Introduction: Although major changes of the immune system have been described in sepsis, it has never been studied whether these may differ in relation to the type of underlying infection or not. This was studied for the first time.

Methods: The statuses of the innate and adaptive immune systems were prospectively compared in 505 patients. Whole blood was sampled within less than 24 hours of advent of sepsis; white blood cells were stained with monoclonal antibodies and analyzed though a flow cytometer.

Results: Expression of HLA-DR was significantly decreased among patients with severe sepsis/shock due to acute pyelonephritis and intraabdominal infections compared with sepsis. The rate of apoptosis of natural killer (NK) cells differed significantly among patients with severe sepsis/shock due to ventilator-associated pneumonia (VAP) and hospital-acquired pneumonia (HAP) compared with sepsis. The rate of apoptosis of NKT cells differed significantly among patients with severe sepsis/shock due to acute pyelonephritis, primary bacteremia and VAP/HAP compared with sepsis. Regarding adaptive immunity, absolute counts of CD4-lymphocytes were significantly decreased among patients with severe sepsis/shock due to community-acquired pneumonia (CAP) and intraabdominal infections compared with sepsis. Absolute counts of B-lymphocytes were significantly decreased among patients with severe sepsis/shock due to CAP compared with sepsis.

Conclusions: Major differences of the early statuses of the innate and adaptive immune systems exist between sepsis and severe sepsis/shock in relation to the underlying type of infection. These results may have a major impact on therapeutics.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Expression of HLA-DR on monocytes and rate of apoptosis of monocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the underlying infection. Patients are divided according to sepsis severity. Double asterisks denote statistically significant differences within the same underlying infection between sepsis and severe sepsis/shock after adjustment for multiple comparisons. CAP: community-acquired pneumonia; HAP: hospital-acquired pneumonia; SE: standard error; VAP: ventilator-associated pneumonia.
Figure 2
Figure 2
Absolute counts and rates of apoptosis of NK cells and of NKT lymphocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the underlying infection. Patients are divided according to sepsis severity. Single asterisk denotes a statistically significant difference between underlying infections after adjustment for multiple comparisons. Double asterisks denote statistically significant differences within the same underlying infection between sepsis and severe sepsis/shock after adjustment for multiple comparisons. CAP: community-acquired pneumonia; HAP: hospital-acquired pneumonia; NK: natural killer; SE: standard error; VAP: ventilator-associated pneumonia.
Figure 3
Figure 3
Absolute counts and rates of apoptosis of CD4- and of CD8-lymphocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the underlying infection. Patients are divided according to sepsis severity. Single asterisk denotes statistically a significant difference between underlying infections after adjustment for multiple comparisons. Double asterisks denote statistically significant differences within the same underlying infection between sepsis and severe sepsis/shock after adjustment for multiple comparisons. CAP: community-acquired pneumonia; HAP: hospital-acquired pneumonia; SE: standard error; VAP: ventilator-associated pneumonia.
Figure 4
Figure 4
Absolute counts of B-lymphocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the underlying infection. Patients are divided according to sepsis severity. Double asterisks denote statistically significant differences within the same underlying infection between sepsis and severe sepsis/shock after adjustment for multiple comparisons. CAP: community-acquired pneumonia; HAP: hospital-acquired pneumonia; SE: standard error; VAP: ventilator-associated pneumonia.
Figure 5
Figure 5
Expression of HLA-DR on monocytes and rate of apoptosis of monocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the implicated pathogens. Patients are divided according to sepsis severity. Single asterisk denotes a statistically significant difference between underlying infections after adjustment for multiple comparisons. Double asterisks denote statistically significant differences within the same underlying infection between sepsis and severe sepsis/shock after adjustment for multiple comparisons. SE: standard error.
Figure 6
Figure 6
Absolute counts and rates of apoptosis of NK cells and of NKT lymphocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the implicated pathogens. Patients are divided according to sepsis severity. NK: natural killer; SE: standard error.
Figure 7
Figure 7
Absolute counts and rates of apoptosis of CD4- and of CD8-lymphocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the implicated pathogens. Patients are divided according to sepsis severity. SE: standard error.
Figure 8
Figure 8
Absolute counts of B-lymphocytes within the first 24 hours of diagnosis among patients with sepsis in relation to the implicated pathogens. Patients are divided according to sepsis severity. SE: standard error.
See this image and copyright information in PMC

Comment in

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Wenzel RP. Treating sepsis. N Engl J Med. 2002;347:966–967. doi: 10.1056/NEJMp020096. - DOI - PubMed
    1. Vincent JL, Sun Q, Dubois MJ. Clinical trials of immunomodulatory therapies in severe sepsis and septic shock. Clin Infect Dis. 2002;34:1084–1093. doi: 10.1086/339549. - DOI - PubMed
    1. Hotchkiss RS, Osmon SB, Chang KC, Wagner TH, Coopersmith CM, Karl IE. Accelerated lymphocyte death in sepsis occurs by both the death receptor and mitochondrial pathways. J Immunol. 2005;174:5110–5118. - PubMed
    1. Unsinger J, Herdon JM, Davis CG, Muenzer JT, Hotchkiss RS, Ferguson TA. The role of TCR engagement and activation-induced cell death in sepsis-induced T cell apoptosis. J Immunol. 2006;177:7968–7973. - PubMed
    1. Pinheiro da Silva F, Chiamolera M, Charles N, Kanamaru Y, Velasco IT, Benhamou M, Monteiro RC. B lymphocytes undergo apoptosis because of FCγRIIB stress response to infection: a novel mechanism of cell death in sepsis. Shock. 2006;25:61–65. doi: 10.1097/01.shk.0000196496.72553.78. - DOI - PubMed

Publication types

Substances