Dengue virus 3 genotype I in Aedes aegypti mosquitoes and eggs, Brazil, 2005-2006

Abstract

Dengue virus type 3 genotype I was detected in Brazil during epidemics in 2002-2004. To confirm this finding, we identified this virus genotype in naturally infected field-caught Aedes aegypti mosquitoes and eggs. Results showed usefulness of virus investigations in vectors as a component of active epidemiologic surveillance.

Figure 1

MosquiTRAP version 2.0 (Ecovec Ltd., Belo Horizonte, Brazil) (A), BG-Sentinel trap (Biogents, Regensburg, Germany) (B), and an ovitrap (C) used for obtaining mosquitoes in the northwestern borough of Belo Horizonte, Minas Gerais, Brazil.

Figure 2

Phylogenetic tree of dengue type 3 serotypes and sequences from Aedes aegypti mosquitoes and larvae obtained in Belo Horizonte, Minas Gerais, Brazil. The tree is based on a 434-nt sequence of the capsid–premembrane gene and was generated by using neighbor-joining analysis with the Tamura-Nei model in MEGA4.1 software (Arizona State University, Tempe, AZ, USA). Numbers to the left of the nodes are bootstrap values (1,000 replicates) in support of the grouping to the right. Numbers in parentheses are GenBank accession numbers. Diamonds indicate viruses sequenced in this study.