IDH1 and IDH2 mutation studies in 1473 patients with chronic-, fibrotic- or blast-phase essential thrombocythemia, polycythemia vera or myelofibrosis

Abstract

In a multi-institutional collaborative project, 1473 patients with myeloproliferative neoplasms (MPN) were screened for isocitrate dehydrogenase 1 (IDH1)/IDH2 mutations: 594 essential thrombocythemia (ET), 421 polycythemia vera (PV), 312 primary myelofibrosis (PMF), 95 post-PV/ET MF and 51 blast-phase MPN. A total of 38 IDH mutations (18 IDH1-R132, 19 IDH2-R140 and 1 IDH2-R172) were detected: 5 (0.8%) ET, 8 (1.9%) PV, 13 (4.2%) PMF, 1 (1%) post-PV/ET MF and 11 (21.6%) blast-phase MPN (P<0.01). Mutant IDH was documented in the presence or absence of JAK2, MPL and TET2 mutations, with similar mutational frequencies. However, IDH-mutated patients were more likely to be nullizygous for JAK2 46/1 haplotype, especially in PMF (P=0.04), and less likely to display complex karyotype, in blast-phase disease (P<0.01). In chronic-phase PMF, JAK2 46/1 haplotype nullizygosity (P<0.01; hazard ratio (HR) 2.9, 95% confidence interval (CI) 1.7-5.2), but not IDH mutational status (P=0.55; HR 1.3, 95% CI 0.5-3.4), had an adverse effect on survival. This was confirmed by multivariable analysis. In contrast, in both blast-phase PMF (P=0.04) and blast-phase MPN (P=0.01), the presence of an IDH mutation predicted worse survival. The current study clarifies disease- and stage-specific IDH mutation incidence and prognostic relevance in MPN and provides additional evidence for the biological effect of distinct JAK2 haplotypes.

Figure 1

High-resolution melting (HRM) normalized and temperature-shifted difference plot for IDH1 (a) and IDH2 (b) and corresponding sequences (c and d).

Figure 2

Survival curves of 111 patients with chronic-phase primary myelofibrosis stratified by their isocitrate dehydrogenase (IDH) mutational status (a), cytogenetic risk (b), JAK2 46/1 haplotype status (c) or International Prognostic Scoring System risk category (d).

Figure 3

Survival curves of patients with blast-phase primary myelofibrosis stratified by their isocitrate dehydrogenase (IDH) mutational (a; n=27 including 8 mutated cases) or JAK2 46/1 haplotype (b; n=23 including 8 nullizygous cases) status.

Figure 4

Survival curves of 43 patients with blast-phase myeloproliferative neoplasm stratified by their isocitrate dehydrogenase (IDH) mutational status.