Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Mar;137(3):551-5.
doi: 10.1007/s00432-010-0906-8. Epub 2010 May 28.

Comparative study of stromal metalloproteases expression in patients with benign hyperplasia and prostate cancer

Safwan Escaff  1 , Jesús M Fernández, Luis O González, Aurelio Suárez, Salomé González-Reyes, José M González, Francisco J Vizoso
Affiliations

Comparative study of stromal metalloproteases expression in patients with benign hyperplasia and prostate cancer

Safwan Escaff et al. J Cancer Res Clin Oncol. 2011 Mar.

Abstract

Purpose: The aim of the present work was to perform a comparative study of stromal cell expressions of MMPs and TIMPs between benign and malignant prostate tissues.

Methods: An immunohistochemical study was performed using specific antibodies against metalloproteases (MMPs) -1, -2, -7, 9, 11, 13, 14 and their tissue inhibitors (TIMPs) -1, 2 and 3, on prostate specimens from 133 patients with clinical localized prostate carcinoma and from 50 patients with BPH.

Results: Our results showed higher percentages of expressions of MMPs and TIMPs by fibroblasts or by mononuclear inflammatory cells (MICs) in prostate carcinomas compared to these cells in BPH. The detection of MMP-2 expression by stromal fibroblasts and/or MMP-2, 9 and TIMP-3 expression by stromal MICs was associated with a 100% of specificity for diagnoses of prostate cancer. We found that the combination of MMP-2 expression by fibroblasts and/or MMP-9 by MICs and/or TIMP-2 by MICs yielded a sensitivity of 47.4%.

Conclusions: Despite of a limited sensitivity (50%), the combination of MMP-TIMPs expression in stromal cells (MMP-2 by fibroblasts and TIMPs by MICs) in our study provided a specificity of 100% for prostate cancer diagnosis.

PubMed Disclaimer

Conflict of interest statement

The authors indicated no potential conflicts of interest.

Figures

Fig. 1
Fig. 1
Examples of immunohistochemical staining. a: 400×; positive expression of MMP-7 by tumour cells. b: 400×; positive expression of MMP-13 by tumour cells. c: 400×; positive expression of TIMP-3 by stromal fibroblasts and d: 400×; positive expression of TIMP-2 by MIC
See this image and copyright information in PMC

References

    1. Bhowmick NA, Moses HL (2005) Tumor-stroma interactions. Curr Opin Genet Dev 15:97–101 - PMC - PubMed
    1. Cheng L, Jones TD, Pan CX, Barbarin A, Eble JN, Koch MO (2005) Anatomic distribution and pathologic characterization of small-volume prostate cancer (< 0.5 ml) in whole-mount prostatectomy specimens. Mod Pathol 18:1022–1026 - PubMed
    1. Cornelius LA, Nehring LC, Harding E et al (1998) Matrix metalloproteinases generate angiostatin: effects on neovascularization. J Immunol 161:6845–6852 - PubMed
    1. Egeblad M, Werb Z (2002) New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer 2:161–174 - PubMed
    1. Epstein JI, Walsh PC, Carmichael M, Brendler CB (1994) Pathologic and clinical findings to predict tumor extent of nonpalpable (stage T1c) prostate cancer. Jama 271:368–374 - PubMed

Publication types

Substances