Bacterial community in the personal-use composting reactor revealed by isolation and cultivation-independent method

Abstract

Composting is an efficient and cost-effective process for organic waste treatment. In order to expand our knowledge regarding microorganisms and their roles in the composting process, bacterial community structures in the personal-use composting reactor were examined by isolation and 16S rDNA clone analysis (cultivation-independent method). The results of 16S rDNA clone analysis showed that populations of the Bacillaceae family (such as Bacillus spp., Cerasibacillus spp., Gracilibacillus spp.), dominate (98%). By using cultivation method, a total of four species including one novel species (Ureibacillus thermosphaericus, Geobacillus thermoglucosidasius, G. toebii and Thermobacillus composti) were isolated, and were classified into the order Bacillales corresponding to the result of 16S rDNA clone analysis. However, most species detected by clone analysis have not been cultivated, and may be viable but non-culturable VBNC species implying symbiotic interactions among the microorganisms.