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Comparative Study
. 2010 Jun;120(6):1188-94.
doi: 10.1002/lary.20884.

Interspecies comparison of mucosal wave properties using high-speed digital imaging

Michael F Regner  1 , Mark J Robitaille, Jack J Jiang
Affiliations
Comparative Study

Interspecies comparison of mucosal wave properties using high-speed digital imaging

Michael F Regner et al. Laryngoscope. 2010 Jun.

Abstract

Objectives/hypothesis: The purpose of this study was to compare the vocal fold vibratory characteristics of ex vivo bovine, canine, ovine, and porcine larynges to human male and female vocal fold vibrations to determine the best model organism for laryngeal studies concerning vibratory and kinetic characteristics.

Study design: Prospective experimental.

Methods: High-speed videos of phonation were gathered at 4,000 frames per second (fps) in the animal models and human high-speed endoscopy data were gathered at 2,000 fps. Videos were converted into kymograms, and the amplitude, oscillation frequency, and phase difference of vocal fold vibration were measured.

Results: No statistically significant differences were found with respect to frequency, amplitude, or phase difference between canines and humans. Porcines were not significantly different from human females but did have an oscillation frequency significantly different from human males. Ovine vibrational amplitudes were significantly different from humans, and bovine frequency and amplitude differed significantly from humans.

Conclusions: Canine and porcine larynges are the most appropriate model specimens for laryngeal studies contingent on vibratory or kinetic properties of phonation.

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Figures

Figure 1
Figure 1
Diagram of the excised larynx phonation system.
Figure 2
Figure 2
The procedure for mucosal wave parameter extraction. High-speed digital images of the glottal plane of phonating excised larynges provided pixels lines from the glottal midline, which were plotted as a function of time to produce a kymograph. Edge extraction methods allowed sinusoidal regressions to be calculated for the left and right superior and inferior vocal fold margins. An idealized kymograph is shown for two glottal cycles, where the origin of the visually detectable glottis is shown to be a function of the inferior and superior glottal widths.
Figure 3
Figure 3
The oscillation frequency across species.
Figure 4
Figure 4
The vibratory amplitude across species. Amplitude is plotted on a logarithmic scale.
Figure 5
Figure 5
The superior-inferior oscillation phase difference across species.
See this image and copyright information in PMC

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