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. 2010 Jun;125(6):1407-1409.e1.
doi: 10.1016/j.jaci.2010.03.037.

Direct ex vivo analysis of allergen-specific CD4+ T cells

William W Kwok, Michelle Roti, Jonathan H Delong, Venus Tan, Erik Wambre, Eddie A James, David Robinson

Direct ex vivo analysis of allergen-specific CD4+ T cells

William W Kwok et al. J Allergy Clin Immunol. 2010 Jun.
No abstract available

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Figures

FIG 1
FIG 1
Ex vivo staining of Fel d 1 CD4+ T cells with tetramers. A, PBMCs from subjects with cat allergy were stained with phycoerythrin-labeled Fel d 1 tetramers (Tet) followed by the anti-phycoerythrin bead enrichment protocol. Each panel shows a representative staining result from a subject with cat allergy with the indicated HLA. The frequencies of Fel d 1 CD4+ T cells (per million) were as indicated. B, Staining of PBMCs from subjects without allergy with Fel d 1 tetramers using the magnetic bead enrichment protocol. Each panel shows a representative staining result from a subject without allergy with the indicated HLA. C, Phenotypes of Fel d 1–specific CD4+ T cells. PBMCs of a DR0401 subject with cat allergy were stained with DR0401/Fel d 1 tetramer ex vivo and a panel of antibodies. D, Comparison of phenotypes of Fel d 1 T cells (●) and total CD4+ T cells (Δ; total of 5 subjects). A Student t test was used in the statistical analysis. *P < .01. E, Cytokine profiles of Fel d 1–reactive CD4+ T cells. PBMCs from subjects with cat allergy with the indicated HLA were stained with tetramers using the anti-phycoerythrin bead enrichment protocol and assayed for IL-5 and IFN-γ.
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