Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2010 Jun 1;15(3):883-900.
doi: 10.2741/3652.

Replication protein A: directing traffic at the intersection of replication and repair

Greg G Oakley  1 , Steve M Patrick
Affiliations
Review

Replication protein A: directing traffic at the intersection of replication and repair

Greg G Oakley et al. Front Biosci (Landmark Ed). .

Abstract

Since the initial discovery of replication protein A (RPA) as a DNA replication factor, much progress has been made on elucidating critical roles for RPA in other DNA metabolic pathways. RPA has been shown to be required for DNA replication, DNA repair, DNA recombination, and the DNA damage response pathway with roles in checkpoint activation. This review summarizes the current understanding of RPA structure, phosphorylation and protein-protein interactions in mediating these DNA metabolic processes.

PubMed Disclaimer

Figures

Figure 1
Figure 1
RPA2 phosphorylation sites. N-terminal residues 1-34 are shown. Indicated above the sequence are sites for which phospho-specific antibodies are available. The putative kinases responsible for phosphorylation of specific residues are shown below the sequence.
Figure 2
Figure 2
Sequence alignment of identified and putative peptide sequences that bind to the N-terminal domain of RPA1.
See this image and copyright information in PMC

References

    1. Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L. Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. Nature. 1997;385:176–181. - PubMed
    1. Bochkarev A, Bochkareva E, Frappier L, Edwards AM. The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding. Embo J. 1999;18:4498–504. - PMC - PubMed
    1. Murzin AG. OB (oligonucleotide/oligosaccharide binding)-fold: common structural and functional solution for non-homologous sequences. Embo J. 1993;12:861–7. - PMC - PubMed
    1. Philipova D, Mullen JR, Maniar HS, Lu J, Gu C, Brill SJ. A hierarchy of SSB protomers in replication protein A. Genes Dev. 1996;10:2222–2233. - PubMed
    1. Brill SJ, Bastin-Shanower S. Identification and characterization of the fourth single-stranded-DNA binding domain of replication protein A. Mol.Cell Biol. 1998;18:7225–7234. - PMC - PubMed

Publication types

LinkOut - more resources