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. 2010 Aug 24;22(32):3593-7.
doi: 10.1002/adma.201000307.

Acetal-modified dextran microparticles with controlled degradation kinetics and surface functionality for gene delivery in phagocytic and non-phagocytic cells

Joel A Cohen  1 , Tristan T Beaudette, Jessica L Cohen, Kyle E Broaders, Eric M Bachelder, Jean M J Fréchet
Affiliations

Acetal-modified dextran microparticles with controlled degradation kinetics and surface functionality for gene delivery in phagocytic and non-phagocytic cells

Joel A Cohen et al. Adv Mater. .
No abstract available

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Figures

Figure 1
Figure 1
a) Scheme for blending Ac-DEX and cationic polymers to synthesize plasmid-loaded microparticles, and subsequent functionalization with a nona-arginine cell-penetrating peptide (CPP). In the center, an SEM image of the dry particles is shown (scale bar = 10 μm). b) Synthesis of cationic poly(β-amino ester) 4 (PBAE) and polyacetal analog 5 (PBAE-PA) used in Ac-DEX particle formulations.
Figure 2
Figure 2
a) Particle zeta-potentials measured pre- (−) and post-modification (+) with a nona-arginine CPP. b) Degradation of particles at pH 7.4 (closed symbols) and pH 5 (open symbols), determined by analysis of released soluble dextran. Data for 30% PBAE-PA blends were nearly identical to their 30% PBAE blend counterparts and were omitted for clarity (see Figure S5a in the Supporting Information). c) Release of plasmid from f-Ac-DEX particles incubated at pH 7.4 or pH 5. Plasmid release profiles for additional particle formulations can be found in Figure S5b in the Supporting Information.
Figure 3
Figure 3
In vitro transfection of a) RAW 264.7 macrophages and b) HeLa cells with plasmid-loaded microparticles (closed symbols), combined with results from a concurrently performed cytotoxicity assay (open symbols). Particles were either not-modified (−) or modifi ed (+) with a nona-arginine CPP.
See this image and copyright information in PMC

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