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Comparative Study
. 2010 Aug;48(8):2991-4.
doi: 10.1128/JCM.02220-09. Epub 2010 Jun 2.

Evaluation of multiple-locus variable-number tandem-repeat analysis for typing a polyclonal hospital-acquired methicillin-resistant Staphylococcus aureus population in an area where such infections are endemic

Belinda Rivero-Pérez  1 , Eduardo Pérez-Roth, Sebastián Méndez-Alvarez
Affiliations
Comparative Study

Evaluation of multiple-locus variable-number tandem-repeat analysis for typing a polyclonal hospital-acquired methicillin-resistant Staphylococcus aureus population in an area where such infections are endemic

Belinda Rivero-Pérez et al. J Clin Microbiol. 2010 Aug.

Abstract

Multiple-locus variable-number tandem-repeat analysis (MLVA) was performed with 292 methicillin-resistant Staphylococcus aureus (MRSA) isolates previously characterized by pulsed-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome mec typing. Quantitative correspondence analyses showed the best correlation between data when an >or=80% cutoff was applied to MLVA. We confirmed the validity of MLVA for identification of related strains in a polyclonal MRSA population.

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Figures

FIG. 1.
FIG. 1.
Classification of the Iberian clone (PFGE-A1/ST247-IA) (A) and the EMRSA-16 clone (PFGE-B1/ST36-II) (B) into different MLVA types and subtypes depending on the cutoff criterion (a, 1 band; b, 80% relatedness; c, 70% relatedness) and relationships to the other PFGE subtypes. The gel images were analyzed with InfoQuest software, version 4.5 (Bio-Rad).
See this image and copyright information in PMC

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