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. 2010 Jul;7(7):512-5.
doi: 10.1038/nmeth.1469. Epub 2010 Jun 6.

Complementary positional proteomics for screening substrates of endo- and exoproteases

Petra Van Damme  1 , An Staes, Silvia Bronsoms, Kenny Helsens, Niklaas Colaert, Evy Timmerman, Francesc X Aviles, Joël Vandekerckhove, Kris Gevaert
Affiliations

Complementary positional proteomics for screening substrates of endo- and exoproteases

Petra Van Damme et al. Nat Methods. 2010 Jul.

Abstract

We describe a positional proteomics approach to simultaneously analyze N- and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing.

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References

    1. Nat Methods. 2005 Mar;2(3):193-200 - PubMed
    1. J Proteome Res. 2007 Dec;6(12):4634-45 - PubMed
    1. Nat Biotechnol. 2001 Mar;19(3):242-7 - PubMed
    1. BMC Proc. 2009 Aug 04;3 Suppl 6:S6 - PubMed
    1. Mol Cell Proteomics. 2002 May;1(5):376-86 - PubMed

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