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. 2010 Sep;54(9):3569-77.
doi: 10.1128/AAC.00057-10. Epub 2010 Jun 7.

Genomewide analysis of divergence of antibiotic resistance determinants in closely related isolates of Acinetobacter baumannii

Mark D Adams  1 , E Ricky Chan, Neil D Molyneaux, Robert A Bonomo
Affiliations

Genomewide analysis of divergence of antibiotic resistance determinants in closely related isolates of Acinetobacter baumannii

Mark D Adams et al. Antimicrob Agents Chemother. 2010 Sep.

Abstract

Multidrug resistance has emerged as a significant concern with infections caused by Acinetobacter baumannii. Ample evidence supports the involvement of mobile genetic elements in the transfer of antibiotic resistance genes, but the extent of variability and the rate of genetic change associated with the acquisition of antibiotic resistance have not been studied in detail. Whole-genome sequence analysis of six closely related clinical isolates of A. baumannii, including four from the same hospital, revealed extensive divergence of the resistance genotype that correlated with observed differences in antimicrobial susceptibility. Resistance genes associated with insertion sequences, plasmids, and a chromosomal resistance gene island all showed variability. The highly dynamic resistance gene repertoire suggests rapid evolution of drug resistance.

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Figures

FIG. 1.
FIG. 1.
Diagram of the resistance island. The AB0057 RI is shown, with boxed areas indicating regions that are absent from AbaR9 in AB056 (solid) and from AbaR10 in AB058 (solid and dashed). (Adapted from reference with permission.)
FIG. 2.
FIG. 2.
Phylogenetic tree of ST15 strains. A phylogenetic tree was made using the neighbor-joining method based on an alignment of DNA sequences from 1,972 open reading frames that are present in each genome assembly. Each branch is supported by at least 90% of 1,000 bootstrap replicates.
See this image and copyright information in PMC

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