doi: 10.1007/BF01935902.
Exchange of tritium from randomly tritiated taurocholate by microbial bile salt oxidoreductases
- PMID: 205428
- DOI: 10.1007/BF01935902
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Exchange of tritium from randomly tritiated taurocholate by microbial bile salt oxidoreductases
Experientia.
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Abstract
Tritium distribution on randomly labelled taurocholate (TC) was estimated at 28%, 4%, 1% and less than 0.5% on the hydrogens opposite the 3alpha-, 7alpha- and 12alpha-OH groups and taurine moiety respectively. Anomalously, C. perfringens 3alpha-hydroxysteroid dehydrogenase (3alpha-HSDH) catalyzed tritium loss of 36% on formation of 7alpha-, 12alpha-dihydroxy-3-keto-5beta-cholanoate, implying additional losses of tritium at other sites by this enzyme.
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