doi: 10.1080/15257771003741422.
Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine
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- PMID: 20544534
- DOI: 10.1080/15257771003741422
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Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine
Nucleosides Nucleotides Nucleic Acids.
2010 Jun.
Abstract
Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.
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