doi: 10.1002/adma.200903612.
Microcrimped collagen fiber-elastin composites
Affiliations
- PMID: 20544890
- PMCID: PMC3213053
- DOI: 10.1002/adma.200903612
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Microcrimped collagen fiber-elastin composites
Adv Mater.
.
No abstract available
Figures
Microcrimping method and scanning electron microscopy of crimped collagen microfibers. A parallel array of hydrated synthetic collagen fibers is placed on a pre-extended polyurethane substrate (a). The polyurethane buttressed-rectangular membrane is pre-extended and clamped over collagen fiber (b). The pre-extension in the substrate and buttressed rectangular membrane is relaxed to generate microcrimp, and the assembly is frozen at -80°C (c). The buttressed-rectangular membrane is removed and the frozen fiber array is transferred to room temperature glutaraldehyde vapor for 24 hr (d). A cooled solution of elastin-mimetic protein is distributed over the microcrimped fiber and pressed into a thin layer with a flat sheet of acrylic (e). After warming to gel the elastin-mimetic protein, the fiber-reinforced layer is separated from the acrylic and polyurethane surfaces (f). SEM of the polyurethane buttressed-rectangular membrane (g), and synthetic collagen fiber arrays templated with 30 and 40% pre-extension after drying and removal of the microridged membrane (h, i). High magnification image of fibers crimped with 40% pre-extension is shown in (j), and a dense sheet of microcrimped fiber in (k). Scale bars 200 μm.
Embedding of microcrimped collagen microfibers within an elastin matrix and fabrication of multilamellar collagen fiber-reinforced elastin sheets. Confocal laser scanning microscopy is used to selectively image the hydrated collagen fibers within a single elastin lamella. The degree of crimp is calculated from the center-line (solid black) and straight-line (dashed white) lengths (a). Fibers in a single lamella sheet pass in and out of the confocal plane due to crimp (b). A 3D reconstruction of the collagen fiber array in (b) demonstrates crimped fiber structure in a single lamellar sheet (c). Multilamellar sheets are fabricated by stacking single sheets, cooling to disrupt physical crosslinks of the protein polymer matrix, and then warming to restore crosslinking and facilitate interlayer bonding. Fibers are oriented parallel to the long axis of the laminate (0°) or alternate at ±25° to the long axis (d). Microcrimping was observed by CLSM in multilamellar sheets (e). Scale bars 200 μm.
Stress-strain behavior of collagen fiber-reinforced uni- and multilamellar elastin composites. (a) Unilamellar sheets reinforced with straight collagen microfibers (○) or microfibers crimped at pre-extension strains of 15% (●) or 30% (■). (b) Responses of uni- (○) and multilamellar (□) composites reinforced with straight collagen microfibers are compared to those of unilamellar (■) and multilamellar (●) composites reinforced with fibers crimped at a pre-extension strain of 30%. (c) Effect of collagen fiber orientation and crimp. The responses of multilamellar elastin composites reinforced with straight collagen fibers oriented at 0° (□) or ±25° (○) are compared to those multilamellar sheets embedded with collagen fibers crimped at a pre-extension strain of 30% and oriented at 0° (■) or ±25° (●). Data presented as mean ± SD from 3 to 9 samples. (d) Uni- (●) and multilamellar (▲) elastin composites reinforced with collagen microfibers crimped at a pre-extension strain of 30% and multilamellar (□) elastin composites embedded with straight fibers oriented at ±25° mimic the mechanical response of glutaraldehyde crosslinked bovine pericardium (○) reported by Sun and colleagues.[24]
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