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. 2010 Sep;163(3):659-61.
doi: 10.1111/j.1365-2133.2010.09892.x. Epub 2010 Jun 9.

History of eczema herpeticum is associated with the inability to induce human β-defensin (HBD)-2, HBD-3 and cathelicidin in the skin of patients with atopic dermatitis

T R Hata, P Kotol, M Boguniewicz, P Taylor, A Paik, M Jackson, M Nguyen, F Kabigting, J Miller, M Gerber, D Zaccaro, B Armstrong, R Dorschner, D Y M Leung, R L Gallo

History of eczema herpeticum is associated with the inability to induce human β-defensin (HBD)-2, HBD-3 and cathelicidin in the skin of patients with atopic dermatitis

T R Hata et al. Br J Dermatol. 2010 Sep.
No abstract available

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Figures

Fig 1
Fig 1
(a)Ability to induce antimicrobial peptides (AMPs) as evidenced by lesional minus nonlesional expression analysed by quantitative reverse transcriptase–polymerase chain reaction (RT-PCR). Skin biopsy samples from lesional and nonlesional skin of subjects were collected and analysed for expression of AMPs. Values of the AMPs were normalized to the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and the data plotted are presented as relative AMP expression. Differences separated by diagnostic group reveal cathelicidin expression in patients with atopic dermatitis (AD) with a history of eczema herpeticum (ADEH) to be significantly depressed compared with patients with AD (P = 0.014) or psoriasis (P = 0.001). (b) Ability to induce human β-defensin (HBD)-2 as evidenced by lesional minus nonlesional expression, analysed by quantitative RT-PCR and normalized to the housekeeping gene GAPDH reveal HBD-2 to be significantly suppressed in patients with ADEH in comparison to those with AD (P = 0.003) or psoriasis (P < 0.001). (c) Ability to induce HBD-3 as evidenced by lesional and nonlesional differences, analysed by quantitative RT-PCR and normalized to the housekeeping gene GAPDH reveal HBD-3 expression in patients with ADEH to be depressed compared with that in patients with psoriasis (P = 0.003), while showing a trend towards lower levels than subjects with AD (P = 0.06). (a–c) Differences were calculated using the ΔΔCt method (see Supporting information).
Fig 2
Fig 2
(a) Nonlesional skin values for cathelicidin analysed by quantitative reverse transcriptase–polymerase chain reaction (RT-PCR) and normalized to the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) revealed no significant differences between the four groups. (b) Analysis of human β-defensin (HBD)-2 in nonlesional skin revealed significantly higher HBD-2 levels in subjects with atopic dermatitis (AD) with a history of eczema herpeticum (ADEH) compared with patients with AD (P < 0.001) or psoriasis (P < 0.001), or normal control subjects (P = 0.001). (c) Analysis of HBD-3 in nonlesional skin revealed significantly higher HBD-3 levels in subjects with ADEH compared with those with AD (P = 0.002) or psoriasis (P = 0.003). CATH, cathelicidin; NonLes, nonlesional; Psor, psoriasis; Cont, control.
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References

    1. Beck LA, Boguniewicz M, Hata T, et al. Phenotype of atopic dermatitis subjects with a history of eczema herpeticum. J Allergy Clin Immunol. 2009;124:260–9. 269.e1–7. - PMC - PubMed
    1. Ong PY, Ohtake T, Brandt C, et al. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N Engl J Med. 2002;347:1151–60. - PubMed
    1. Nomura I, Goleva E, Howell MD, et al. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J Immunol. 2003;171:3262–9. - PubMed
    1. Howell MD, Wollenberg A, Gallo RL, et al. Cathelicidin deficiency predisposes to eczema herpeticum. J Allergy Clin Immunol. 2006;117:836–41. - PMC - PubMed
    1. Schauber J, Dorschner RA, Coda AB, et al. Injury enhances TLR2 function and antimicrobial peptide expression through a vitamin D-dependent mechanism. J Clin Invest. 2007;117:803–11. - PMC - PubMed

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