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. 2010 Sep;54(9):3714-6.
doi: 10.1128/AAC.00358-10. Epub 2010 Jun 14.

Amodiaquine resistance in Plasmodium falciparum malaria in Afghanistan is associated with the pfcrt SVMNT allele at codons 72 to 76

Khalid Beshir  1 , Colin J Sutherland, Ioannis Merinopoulos, Naeem Durrani, Toby Leslie, Mark Rowland, Rachel L Hallett
Affiliations

Amodiaquine resistance in Plasmodium falciparum malaria in Afghanistan is associated with the pfcrt SVMNT allele at codons 72 to 76

Khalid Beshir et al. Antimicrob Agents Chemother. 2010 Sep.

Abstract

Mutations in the Plasmodium falciparum genes pfcrt and pfmdr1 are selected by amodiaquine treatment in Africa. To examine the importance of these mutations in amodiaquine-treated Asian parasites, we determined pre- and posttreatment genotypes for amodiaquine treatment failures from a clinical trial in Afghanistan. The pfcrt codon 72 to 76 haplotype SVMNT was present in all samples tested, both before and after treatment. Amodiaquine did not clearly select for any pfmdr1 genotype, but a novel mutation, pfmdr1 N86F, was detected in four samples. We provide in vivo data to support the in vitro correlation between pfcrt SVMNT and increased resistance to the metabolite of amodiaquine.

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Figures

FIG. 1.
FIG. 1.
Prevalences of pfmdr1 haplotypes at amino acids 86, 184, 1034, 1042, and 1246 before and after treatment with AQ.
See this image and copyright information in PMC

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