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. 2010 May;22(2):149-55.
doi: 10.5021/ad.2010.22.2.149. Epub 2010 May 17.

Epidemiologic Study of Malassezia Yeasts in Seborrheic Dermatitis Patients by the Analysis of 26S rDNA PCR-RFLP

Byung Ho Oh  1 , Yang Won Lee, Yong Beom Choe, Kyu Joong Ahn
Affiliations

Epidemiologic Study of Malassezia Yeasts in Seborrheic Dermatitis Patients by the Analysis of 26S rDNA PCR-RFLP

Byung Ho Oh et al. Ann Dermatol. 2010 May.

Abstract

Background: This case-control study concerns a molecular biological method based on the data gathered from a group of Korean subjects to examine the distribution of Malassezia yeasts in seborrheic dermatitis (SD) patients. Cultures for Malassezia yeasts were taken from the foreheads, cheeks and chests of 60 patients with SD and in 60 healthy controls of equivalent age.

Objective: The purpose of this study is to identify the relationship between certain species of Malassezia and SD. This was done by analyzing the differences in the distribution of Malassezia species in terms of age and body parts of the host with healthy controls.

Methods: 26S rDNA PCR-RFLP, a fast and accurate molecular biological method, was used to overcome the limits of morphological and biochemical methods.

Results: The positive Malassezia culture rate was 51.7% in patients with SD, which was lower than that of healthy adults (63.9%). M. restricta was dominant in patients with SD (19.5%). Likewise, M. restricta was identified as a common species (20.5%) in healthy controls. In the ages 31~40, M. restricta was found to be the most common species (31.6%) among SD patients.

Conclusion: According to the results of the study, the most frequently isolated species was M. restricta (19.5%) in patients with SD. There was no statistically significant difference in the distribution of Malassezia species between the SD patients and healthy control groups.

Keywords: 26S rDNA PCR-RFLP; Malassezia yeasts; Seborrheic dermatitis.

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Figures

Fig. 1
Fig. 1
PCR-RFLP patterns of 26S rDNA PCR digested with Hha I (A), BtsC I (B) of 11 Malassezia standard strains. Lanes: M: molecular marker, 1: M. furfur (KCTC 7743), 2: M. sympodialis (KCTC 7985), 3: M. globosa (CBS 7966), 4: M. restricta (KCTC 7848), 5: M. slooffiae (KCTC 17431), 6: M. pachydermatis (KCTC 17008), 7: M. japonica (CBS 9432), 8: M. nana (JCM 12085), 9: M. dermatis (JCM 11348), 10: M. obtusa (KCTC 7847), 11: M. yamatoensis (CBS 9725).
Fig. 2
Fig. 2
PCR-RFLP patterns of 26S rDNA PCR digested with Hha I (A), BtsC I (B) of Malassezia yeasts from seborrheic dermatitis patients and healthy controls. Lanes: M: molecular marker, 1: M. restricta, 2: M. globosa, 3: M. sympodialis, 4: M. furfur, 5: M. dermatis, 6: M. slooffiae (from healthy control), 7: M. obtusa (from healthy control).
Fig. 3
Fig. 3
Identified Malassezia species from seborrheic dermatitis group compared with healthy control group, by ages.
Fig. 4
Fig. 4
Identified Malassezia species from seborrheic dermatitis group compared with healthy control group, by body sites.
See this image and copyright information in PMC

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