Antioxidants, Oxyrase, and mitochondrial uncoupler 2,4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival

Abstract

Various antioxidant strategies such as supplementation of antioxidants, limiting oxygen concentration with Oxyrase, and reducing reactive oxygen species through mild mitochondrial uncoupling had statistically significant beneficial effects on sperm cryopreservation from rhesus monkeys with low cryoresistant ejaculates. Individuals or species that have higher sensitivity to cryodamage may derive the most benefit from these treatments.

Fig. 1

Post-thaw motility of rhesus monkey sperm samples frozen in TEY-3% glycerol with additives (closed circles) of 200 U/ml superoxide dimutase (SOD), 200 U/ml catalase (CAT), 0.3 U/ml oxyrase, 10 mM vitamin E (VE), and dinitrophenol (DNP) at the concentration range of 0.01, 0.1, 1, 10, 50 μM vs. controls without these additives (open circles). Controls for VE and DNP additives were 0.1% ethanol solvent control. Males with low post-thaw motility in controls (less than 60% for SOD, CAT, and oxyrase or less than 50% for VE and DNP groups) were shown in Panel (a), and males with high post-thaw motility in controls (equal or above 60% for SOD, CAT, and oxyrase groups or 50% for VE and DNP groups) were shown in Panel (b). Asterisks indicate significant difference between controls and treatment groups.