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Comment
. 2010 Aug;192(16):4086-8.
doi: 10.1128/JB.00573-10. Epub 2010 Jun 18.

The surprising Rut pathway: an unexpected way to derive nitrogen from pyrimidines

Rebecca E Parales  1 , John L Ingraham
Affiliations
Comment

The surprising Rut pathway: an unexpected way to derive nitrogen from pyrimidines

Rebecca E Parales et al. J Bacteriol. 2010 Aug.
No abstract available

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Figures

FIG. 1.
FIG. 1.
The rut gene cluster and proposed pathway for uracil catabolism in E. coli. (A) Genes required for pyrimidine utilization, including rutRABCDEFG and ydfG, which is not colocalized with the rut gene cluster on the E. coli chromosome. Functions of the gene products are indicated below each gene. The gene encoding the uracil transporter protein (rutG) is striped. Genes in black (rutA, rutF, and rutB) encode enzymes that are sufficient to release both ring nitrogen atoms from uracil in vitro. Genes in white (rutCDE and ydfG) encode functions that are required in vivo to stimulate slow spontaneous reactions and/or eliminate toxic intermediates. The product of rutR (shown in gray) represses transcription of the rut operon. Under nitrogen-limiting conditions, transcription is activated by NtrC (not shown) and repression is relieved by uracil (6). (B) Proposed uracil catabolic pathway showing enzymatic and spontaneous steps and intermediates. Spontaneous reactions are shown as dotted arrows; others are shown as solid arrows. Ammonia molecules released from uracil are circled. The remaining by-product, 3-hydroxypropionate, is excreted from cells unused.
See this image and copyright information in PMC

Comment on

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