Stem rust of small grains and grasses caused by Puccinia graminis

Abstract

SUMMARY Stem rust has been a serious disease of wheat, barley, oat and rye, as well as various important grasses including timothy, tall fescue and perennial ryegrass. The stem rust fungus, Puccinia graminis, is functionally an obligate biotroph. Although the fungus can be cultured with difficulty on artificial media, cultures grow slowly and upon subculturing they develop abnormal ploidy levels and lose their ability to infect host plants [Bushnell and Bosacker (1982) Can. J. Bot. 60, 1827-1836]. P. graminis is a typical heteroecious rust fungus with the full complement of five distinct spore stages that occur during asexual reproduction on its gramineous hosts and sexual reproduction that begins in the resting spore stage and culminates on the alternate host, barberry (Berberis spp.). There appears to be little polymorphism for resistance/susceptibility in Berberis species, but complex polymorphisms of resistance/susceptibility and matching virulence/avirulence exist in gene-for-gene relationships between small grain species and the forms of P. graminis that infect them.

Taxonomy: Puccinia graminis is a rust fungus in the phylum Basidiomycota, class Urediniomycetes, order Uredinales, and family Pucciniaceae, which contains 17 genera and approximately 4121 species, of which the majority are in the genus Puccinia[Kirk et al. (2001) Ainsworth and Bisby's Dictionary of the Fungi. Wallingford, UK: CAB International]. Various subdivisions of P. graminis into subspecies, varieties and formae speciales have been proposed based on spore size and host range. Crossing studies and DNA sequence comparisons support the separation of at least two subspecies, but not the proposed separation based on spore size.

Host range: The host range of P. graminis is very broad compared with that of most Puccinia spp.; it includes at least 365 species of cereals and grasses in 54 genera [Anikster (1984) The Cereal Rusts. Orlando, FL: Academic Press, pp. 115-130]. Wheat stem rust, P. graminis f. sp. tritici, was shown to infect 74 species in 34 genera in artificial inoculations of seedlings, but only 28 of those species belonging to eight genera were known to be natural hosts of the fungus. Other formae speciales of P. graminis have narrower host ranges than P. graminis f. sp. tritici. Disease symptoms: Infections in cereals or grasses occur mainly on stems and leaf sheaths, but occasionally they may be found on leaf blades and glumes as well. The first macroscopic symptom is usually a small chlorotic fleck, which appears a few days after infection. About 8-10 days after infection, a pustule several millimetres long and a few millimetres wide is formed by rupture of the host epidermis from pressure of a mass of brick-red urediniospores produced in the infection. These uredinial pustules are generally linear or diamond shaped and may enlarge up to 10 mm long. The powdery masses of urediniospores appear similar to rust spots on a weathered iron surface. With age, the infection ceases production of brick-red urediniospores and produces a layer of black teliospores in their place, causing the stems of heavily infected plants to appear blackened late in the season.