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. 2010:10:48.
doi: 10.1673/031.010.4801.

Study of oostatic peptide uptake and metabolism in developing ovaries of the flesh fly, Neobellieria bullata

Blanka Bennettová  1 , Jirina Slaninová, Vĕra Vlasáková, Jan Hlavácek, Josef Holík, Richard Tykva
Affiliations

Study of oostatic peptide uptake and metabolism in developing ovaries of the flesh fly, Neobellieria bullata

Blanka Bennettová et al. J Insect Sci. 2010.

Abstract

The uptake and metabolism of the oostatic pentapeptide analogue of trypsin modulating oostatic factor (TMOF), H-Tyr-Asp-Pro-Ala-Pro-OH (5P), in ovaries of Neobellieria bullata (Parker) (Diptera: Sarcophagidae) were analyzed during their developmental stages. During selected stages of yolk deposition, the fate of [3HPro(3)]5P after its in vivo injection was compared to its uptake after in vitro incubation of dissected ovaries. The ovaries were analyzed from 30 s to 180 min after incubation. A detection sensitivity of 60-100 fmol of the labeled 5P was achieved using radio-high performance liquid chromatography. While the uptake of the applied radioactivity strongly depended on the stage of vitellogenesis, especially for the in vitro experiment, degradation of 5P was very quick and independent of whether the label was injected or incubated with the ovaries, regardless of the developmental stage of ovaries. No tracers of 5P were detected at 30 s after applying the labeled 5P in all tests.

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Figures

Figure 1.
Figure 1.
Histology of degenerative changes in the flesh fly Neobellieria bullata ovaries after application of oostatic pentapeptide (5P). A: Normal ovary development. B: The inner space of egg chamber filled up with nuclei and cells that originated from follicular epithelium. High quality figures are available online.
Figure 2.
Figure 2.
Radioactivity of ovaries in different vitellogenic stages of development in dependence on time after application in vivo (1, 2) or in vitro (3–6). Stage of development according to King (1970): 1: 7th and 8th; 2: 9th and 10th; 3; 7th; 4: 8th; 5: 9th; 6: 10th. Sets of 10 samples in 3 independent experiments were evaluated for each point, S.D. of the presented means from ± 16% to ± 25%. High quality figures are available online.
Figure 3.
Figure 3.
Lower; radiochromatogram of an extract of ovaries of the 10th stage 5 min after [3HPro3]5P in vitro application; Upper; chromatogram of non-active standards. High quality figures are available online.
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References

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