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. 2010 Jun 23:7:136.
doi: 10.1186/1743-422X-7-136.

Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus strains with P[12] specificity

LaShanda M Long-Croal  1 , Xiaobo Wen, Eileen N Ostlund, Yasutaka Hoshino
Affiliations

Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus strains with P[12] specificity

LaShanda M Long-Croal et al. Virol J. .

Abstract

Background: It is universally acknowledged that genome segment 4 of group A rotavirus, the major etiologic agent of severe diarrhea in infants and neonatal farm animals, encodes outer capsid neutralization and protective antigen VP4.

Results: To determine which genome segment of three group A equine rotavirus strains (H-2, FI-14 and FI-23) with P[12] specificity encodes the VP4, we analyzed dsRNAs of strains H-2, FI-14 and FI-23 as well as their reassortants by polyacrylamide gel electrophoresis (PAGE) at varying concentrations of acrylamide. The relative position of the VP4 gene of the three equine P[12] strains varied (either genome segment 3 or 4) depending upon the concentration of acrylamide. The VP4 gene bearing P[3], P[4], P[6], P[7], P[8] or P[18] specificity did not exhibit this phenomenon when the PAGE running conditions were varied.

Conclusions: The concentration of acrylamide in a PAGE gel affected VP4 gene coding assignment of equine rotavirus strains bearing P[12] specificity.

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Figures

Figure 1
Figure 1
Electrophoretic migration patterns in a 12% PAGE gel of equine rotavirus H-2 strain, H-2 × DS-1 reassortant, and human rotavirus DS-1 strain; equine rotavirus FI-14 strain, FI-14 × DS-1 reassortant and DS-1 strain; and equine rotavirus FI-23 strain, FI-23 × DS-1 reassortant, and DS-1 strain. Arrows indicate the VP4 gene (3rd genome segment) of each of the 3 equine parental rotavirus strains.
Figure 2
Figure 2
Electrophoretic migration patterns in a 7.5% PAGE gel of equine rotavirus H-2 strain, H-2 × DS-1 reassortant and human rotavirus DS-1 strain. Arrow indicates the VP4 gene (4th genome segment) of the H-2 strain.
Figure 3
Figure 3
Electrophoretic migration patterns in a 10% PAGE gel of equine rotavirus H-2 strain, H-2 × DS-1 reassortant and human rotavirus DS-1 strain. Arrow indicates the VP4 gene of the H-2 strain. Note the 3rd and 4th genome segments of the H-2 strain comigrate.
Figure 4
Figure 4
Electrophoretic migration patterns in a 15% PAGE gel of equine rotavirus H-2 strain, H-2 × DS-1 reassortant and human rotavirus DS-1 strain. Arrow indicates the VP4 gene (3rd genome segment) of the H-2 strain.
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References

    1. Cheng AC, McDonald JR, Thielman NM. Infectious diarrhea in developed and developing countries. Clin Gastroenterol. 2005;39:757–73. doi: 10.1097/01.mcg.0000177231.13770.07. - DOI - PubMed
    1. Magdesian KG. Neonatal foal diarrhea. Vet Clin North Am Equine Pract. 2005. pp. 295–312. - DOI - PMC - PubMed
    1. Estes MK, Kapikian AZ. In: Fields virology. 5. Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE, editor. Philadelphia, PA: Lippincott Williams and Wilkins; 2007. Rotaviruses; pp. 1917–74.
    1. Saif LJ, Rosen BI, Parwani AVL. In: Viral Infections of the Gastrointestinal Tract. Kapikian AZ, editor. New York: Marcel Dekker; 1994. Animal rotaviruses; pp. 279–367.
    1. Flewett TH, Bryden AS, Davies H. Virus diarrhoea in foals and other animals. Vet Rec. 1975;96:477. - PubMed

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