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. 2010 Aug;105(2):115-21.
doi: 10.1007/s11120-010-9569-1. Epub 2010 Jun 24.

Absorbance changes accompanying the fast fluorescence induction in the purple bacterium Rhodobacter sphaeroides

David Bína  1 , Radek Litvín, Frantisek Vácha
Affiliations

Absorbance changes accompanying the fast fluorescence induction in the purple bacterium Rhodobacter sphaeroides

David Bína et al. Photosynth Res. 2010 Aug.

Abstract

The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in the reaction center and the membrane potential. The results of this study support the view that the membrane potential act predominantly to increase the fluorescence yield. Advantages of the pulsed actinic illumination for study of the operation of the electron transport chain in vivo are discussed.

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