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. 2010 Jul:Chapter 18:Unit 18.15.
doi: 10.1002/0471142727.mb1815s91.

Visualization of kinase activity with FRET-based activity biosensors

Charlene Depry  1 , Jin Zhang
Affiliations

Visualization of kinase activity with FRET-based activity biosensors

Charlene Depry et al. Curr Protoc Mol Biol. 2010 Jul.

Abstract

Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy.

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