Phytoglycoprotein (75 kDa) inhibits expression of interleukin-1beta stimulated by DEHP in human mast cells

Abstract

The purpose of this study is to investigate the inhibitory effect of a glycoprotein (CTB glycoprotein, 75 kDa) isolated from Cudrania tricuspidata Bureau (CTB) on the di-(2-ethylhexyl)phthalate (DEHP) induced expression of allergic-inflammation-related mediators in human mast cells. The changes on the levels of intracellular reactive oxygen species (ROS), mitogen-activated protein kinase (MAPK), transcription factor [nuclear factor (NF)-kappaB], and allergic inflammatory mediators [cyclooxygenase (COX)-2 and interleukin (IL)-1beta] were evaluated using Western blot and RT-PCR. Our results showed that the CTB glycoprotein in the presence of DEHP inhibits the production of intracellular ROS, the phosphorylation of ERK1/2, and p38 MAPK in HMC-1 cells. In addition, the CTB glycoprotein has suppressive effects on the transcriptional activation of NF-kappaB, and on the expression levels of COX-2 and IL-1beta in DEHP-treated HMC-1 cells. In conclusion, the CTB glycoprotein has a strong anti-inflammatory effect on the activities of allergic inflammatory mediators indirectly caused by DEHP in HMC-1 cells.