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. 1978 Apr;75(4):1788-91.
doi: 10.1073/pnas.75.4.1788.

Regulation of adenylate cyclase activity mediated by muscarinic acetylcholine receptors

N M Nathanson, W L Klein, M Nirenberg

Regulation of adenylate cyclase activity mediated by muscarinic acetylcholine receptors

N M Nathanson et al. Proc Natl Acad Sci U S A. 1978 Apr.

Abstract

Carbachol, an activator of muscarinic acetylcholine receptors of NG108-15 hybrid cells, inhibits adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] rapidly and reversibly and slowly evokes a 200-300% increase in adenylate cyclase activity over a period of 24-30 hr. Both the inhibition of adenylate cyclase and the gradual increase in enzyme activity are dependent on muscarinic acetylcholine receptors and the receptor activator. Withdrawal of carbachol results in a gradual return of adenylate cyclase activity to control levels over a period of 6 hr; the half-life for decay of enzyme activity is 1.6 hr. These results show that muscarinic acetylcholine receptors mediate both transient and long-lived effects on adenylate cyclase activity that resemble those of opiates.

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References

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