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. 1991 Apr;57(4):1013-7.
doi: 10.1128/aem.57.4.1013-1017.1991.

Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid

A K Bej  1 , J L DiCesare, L Haff, R M Atlas
Affiliations

Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid

A K Bej et al. Appl Environ Microbiol. 1991 Apr.

Erratum in

  • Appl Environ Microbiol 1991 Aug;57(8):2445

Abstract

A method was developed for the detection of the fecal coliform bacterium Escherichia coli, using the polymerase chain reaction and gene probes, based on amplifying regions of the uid gene that code for beta-glucuronidase, expression of which forms the basis for fecal coliform detection by the commercially available Colilert method. Amplification and gene probe detection of four different regions of uid specifically detected E. coli and Shigella species, including beta-glucuronidase-negative strains of E. coli; no amplification was observed for other coliform and nonenteric bacteria.

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References

    1. Mol Gen Genet. 1985;199(1):101-5 - PubMed
    1. J Clin Microbiol. 1986 Sep;24(3):368-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51 - PubMed
    1. Yale J Biol Med. 1988 Sep-Oct;61(5):389-99 - PubMed
    1. Appl Environ Microbiol. 1989 Feb;55(2):335-9 - PubMed

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