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. 2010 Apr;74(2):149-52.

Genomic analysis of porcine circovirus type-2 isolates in Alberta pigs demonstrating clinical porcine circovirus associated disease (PCVAD)

Leila McIntyre  1 , Mark Chaiyakul, Edward G Clark, Frank Marshall, Markus Czub
Affiliations

Genomic analysis of porcine circovirus type-2 isolates in Alberta pigs demonstrating clinical porcine circovirus associated disease (PCVAD)

Leila McIntyre et al. Can J Vet Res. 2010 Apr.

Abstract
in English, French

Nineteen pigs with clinical signs of porcine circovirus associated diseases (PCVAD) on 5 Alberta pig farms were examined pathologically, including gross pathology, histopathology, and immunohistochemistry. Polymerase chain reaction (PCR) for porcine circovirus type-2 (PCV-2) and sequence analysis was performed on tissue samples of 12 animals. Results showed that new strains of porcine circovirus type-2 genogroup b were present in most pigs that were positive for PCV-2. Furthermore, a mixed infection with PCV-2a and PCV-2b occurred in the liver and lungs of 1 pig. Comparison of whole genome sequences representing known viruses and the newly discovered Alberta viruses revealed mutations distributed over the entire genome of PCV-2; however, sequences encoding for immunodominant epitopes of PCV-2 appear to be unaffected by these mutations.

Dix-neuf porcs présentant des signes cliniques de maladie associée au circovirus (PCVAD) dans 5 fermes de l’Alberta ont été soumis à un examen pathologique, incluant un examen macroscopique, histopathologique et immuno-histochimique. Une réaction d’amplification en chaîne par la polymérase (PCR) pour le circovirus porcin de type 2 (PCV-2) et une analyse de séquence ont été effectuées sur des échantillons de tissus provenant de 12 animaux. Les résultats ont montré que de nouvelles souches de PCV-2 appartenant au génogroupe b étaient présentes chez la majorité des porcs qui étaient positifs pour PCV-2. De plus, une infection mixte avec PCV-2a et PCV-2b s’est produite dans le foie et les poumons de 1 porc. Une comparaison des séquences du génome entier représentant des virus connus et les nouveaux virus découverts en Alberta ont révélé des mutations distribuées sur l’ensemble du génome de PCV-2; toutefois, les séquences codant pour des épitopes immunodominants de PCV-2 ne semblaient pas être affectées par ces mutations.

(Traduit par Docteur Serge Messier)

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