Review
doi: 10.1016/j.ymeth.2010.06.014.
Epub 2010 Jun 26.
In vitro evolution and analysis of HIV-1 LTR-specific recombinases
Affiliations
- PMID: 20600935
- DOI: 10.1016/j.ymeth.2010.06.014
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Review
In vitro evolution and analysis of HIV-1 LTR-specific recombinases
Methods.
2011 Jan.
Abstract
Current antiretroviral therapies would greatly benefit from the concurrent removal of integrated HIV-1 proviral DNA from the patient's cells. In this review, we describe an experimental strategy that allowed the engineering and functional analysis of a HIV-1 LTR-specific recombinase (Tre-recombinase). We furthermore provide protocols that are utilized for the investigation of Tre's antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2(-/-)γc(-/-) mice.
Copyright © 2010 Elsevier Inc. All rights reserved.
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