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. 2010 Jul 5:7:8.
doi: 10.1186/1742-4933-7-8.

Characterization of leukotrienes in a pilot study of older asthma subjects

Sharmilee M Nyenhuis  1 , Elizabeth A Schwantes, Sameer K Mathur
Affiliations

Characterization of leukotrienes in a pilot study of older asthma subjects

Sharmilee M Nyenhuis et al. Immun Ageing. .
No abstract available

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Figures

Figure 1
Figure 1
Sputum cell type differentials. Box plots of sputum cell percentages (1A) and total eosinophil (1B) and neutrophil (1C) number are shown for young subjects (white bars; n = 11), and older subjects (gray bars; n = 6). One young subject had > 80% epithelial cells in the sputum (indicating oral/salivary contamination) and was not included in the sputum differential. Comparisons between the young and older groups were performed with t test analysis or Mann-Whitney rank sum analysis. Statistical significance was defined at p < 0.05. Mac = macrophage; Eos = eosinophil; Neut = neutrophil; Lymph = lymphocyte; Median: ----- (solid)
Figure 2
Figure 2
In vivo and in vitro leukotriene levels. Comparisons between the young and older groups were performed with t test analysis or Mann-Whitney rank sum analysis. Statistical significance was defined at p < 0.05. White bars: young subjects; Gray bars: older subjects Mean: - - -(dashed) Median: ----- (solid). 2A and 2B. Sputum was processed and LTB4 and CysLT levels were measured using the LTB4 and CysLT competitive EIA kit, respectively (Cayman Chemical, Ann Arbor, MI). Sputolysin (Calbiochem) was added to all assay standards to represent an equivalent amount in the diluted sputum samples and run in duplicate. Standard curves for LTB4 and CysLT were performed both with and without Sputolysin to ensure an equivalent slope was obtained. Young: n = 11 (One young subject had > 80% epithelial cells in the sputum and was not included in the biochemical analysis.); Older: n = 6. 2C and 2D. Peripheral blood neutrophils and eosinophils were isolated and stimulated with calcium ionophore (A23187) for 60 min at 37°C. LTB4 and LTC4 were measured by competitive EIA kit (Cayman Chemical, Ann Arbor, MI). Young: n = 12; Older: n = 6
Figure 3
Figure 3
Production of GM-CSF in PBMCs; Peripheral blood mononuclear cells (PBMCs) were either not stimulated or stimulated with LPS (0.4 μg/mL) and supernatants analyzed by ELISA for GM-CSF. Comparisons between the young and older groups were performed with t test analysis. Statistical significance was defined at p < 0.05. White bars: young subjects (n = 12); Gray bars: older subjects (n = 6); Mean: - - - (dashed) Median: ----- (solid)
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