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Review
. 2010 Jun;8 Suppl 3(Suppl 3):s48-52.
doi: 10.2450/2010.008S.

Changes in band 3 structure as determinants of erythrocyte integrity during storage and survival after transfusion

Giel J C G M Bosman  1 , Mark Stappers, Vera M J Novotný
Affiliations
Review

Changes in band 3 structure as determinants of erythrocyte integrity during storage and survival after transfusion

Giel J C G M Bosman et al. Blood Transfus. 2010 Jun.
No abstract available

Keywords: band 3; erythrocyte; storage; transfusion.

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Figures

Figure 1
Figure 1
Immunoblot analysis of membrane proteins from erythrocytes of various storage times with anti-band 3 antibodies. Immunoblot analysis of erythrocytes stored for the indicated periods (0, 16, 29, 36 days) was performed with antibodies against the membrane domain of band 3. +, membranes isolated after incubation with papain. A, increased breakdown and aggregation during storage; B, increased susceptibility to proteolytic breakdown with storage. The arrows indicate the position of intact band 3.
Figure 2
Figure 2
The effect of treatment of erythrocytes during storage on the capacity to bind autologous IgG. Erythrocytes of various storage periods were subjected to incubation with hyperosmotic medium inducing cell shrinkage, with hydroperoxide inducing oxidation, with ionomycin inducing an increase in intracellular calcium concentration, and with SITS inducing crosslinking of band 3 molecules. After these treatments the cells were incubated with autologous plasma and IgG binding was determined by flowcytometry using FITC-labeled anti-human IgG (Cappel). Hyperosmotic shock and SITS induce an increase in IgG binding starting at three weeks of storage.
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References

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