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. 2010 Jul;4(3):287-92.

Effect of different polymerization methods on the cytotoxicity of dental composites

Nilufer Celebi Beriat  1 , Ahmet Atila Ertan, Senay Canay, Aylin Gurpinar, Mehmet Ali Onur
Affiliations

Effect of different polymerization methods on the cytotoxicity of dental composites

Nilufer Celebi Beriat et al. Eur J Dent. 2010 Jul.

Abstract

Objectives: The aim of this study was to compare the cytotoxic effects of various dental composites polymerized with two different curing units.

Methods: Disc-shaped test samples of composites Filtek Z250, Filtek A110, Filtek P60, Filtek Supreme, and SDI Rok were polymerized using one quartz tungsten halogen (QTH) and one light emitting diode (LED) light curing unit (LCU), namely Optilux 501 (QTH) and Elipar Freelight 2 (LED). L-929 mouse fibroblast cultures (3x10(4) cells/ml) were incubated with the samples in 96 well culture plates for evaluation after 8, 24, 48, 72 h. At the end of each period, the cells were counted and examined under a light microscope, and a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was performed. The degree of cytotoxicity for each sample was determined according to the reference value represented by the cells in a control group (a culture without sample).

Results: A significant 3 factor interaction occurred among LCUs, composites, and time factors (P<.005). In general, the test materials cured with the LED LCU demonstrated higher cell survival rates when compared with those cured with halogen LCUs.

Conclusions: This study shows that polymerization of dental composites with a light emitting diode LCU positively influences the L-929 mouse fibroblast cell viability.

Keywords: Cytotoxicity; Dental composite; Light curing units.

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Figures

Figure 1.
Figure 1.
Cell viability of the samples polymerized by LED and QTH light curing units.
Figure 2.
Figure 2.
Cell viability of the samples polymerized by LED.
Figure 3.
Figure 3.
Cell viability of the samples polymerized by QTH.
See this image and copyright information in PMC

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