Lineage analysis of stem cells

Excerpt

Clonal marking methods represent one of the most powerful tools available for identifying stem cells and analyzing their function. Derived from classical approaches in cell lineage tracing during development, we review modern cell lineage methods and describe how they are used to identify stem cells and their niches. We discuss key considerations a stem cell researcher must be aware of to avoid some of the common pitfalls of lineage studies. Finally, using basic knowledge of the target tissue and the guidelines discussed herein, we explain how raw lineage information can be converted into sound conclusions about the number, location, behavior and regulation of tissue stem cells.