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. 2010 Jun 27;6(4):350-60.
doi: 10.7150/ijbs.6.350.

Differential transcriptional analysis between red and white skeletal muscle of Chinese Meishan pigs

Yang Li  1 , Zaiyan Xu, Hongying Li, Yuanzhu Xiong, Bo Zuo
Affiliations

Differential transcriptional analysis between red and white skeletal muscle of Chinese Meishan pigs

Yang Li et al. Int J Biol Sci. .

Abstract

In order to better understand and elucidate the major determinants of red and white muscle phenotypic properties, the global gene expression profiling was performed in white (longissimus doris) and red (soleus) skeletal muscle of Chinese Meishan pigs using the Affymetrix Porcine Genechip. 550 transcripts at least 1.5-fold difference were identified at p < 0.05 level, with 323 showing increased expression and 227 decreased expression in red muscle. Quantitative real-time PCR validated the differential expression of eleven genes (alpha-Actin, ART3, GATA-6, HMOX1, HSP, MYBPH, OCA2, SLC12A4, TGFB1, TGFB3 and TNX). Twenty eight signaling pathways including ECM-receptor interaction, focal adhesion, TGF-beta signaling pathway, MAPK signaling pathway, Wnt signaling pathway, mTOR signaling pathway, insulin signaling pathway and cell cycle, were identified using KEGG pathway database. Our findings demonstrate previously unrecognized changes in gene transcription between red and white muscle, and some potential cascades identified in the study merit further investigation.

Keywords: Affymetrix; Differential transcriptional analysis; Longissimus doris; Pig; Soleus.

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Conflict of interest statement

Conflict of Interest: The authors have declared that no conflict of interest exists.

Figures

Figure 1
Figure 1
Expression of four MyHC isoforms in longissimus doris and soleus mRNA by qRT-PCR. The data presented in Y-axis were calculated using the expression values of 2−ΔΔCt of three pigs and expressed as means ± s.d.
Figure 2
Figure 2
Validation of differentially expressed genes between longissimus doris (LD) and soleus (SE) by qRT-PCR. The data presented in Y-axis indicated the relative mRNA expression of both microarray (M) and qRT-PCR (Q) and expressed as means of three pigs ± s.d. The correlation coefficient (R) and the corresponding significance value (P) were shown above their respective columns.
Figure 2
Figure 2
Validation of differentially expressed genes between longissimus doris (LD) and soleus (SE) by qRT-PCR. The data presented in Y-axis indicated the relative mRNA expression of both microarray (M) and qRT-PCR (Q) and expressed as means of three pigs ± s.d. The correlation coefficient (R) and the corresponding significance value (P) were shown above their respective columns.
Figure 3
Figure 3
Gene pathway network about the differential expressed genes. The differential expressed genes and the corresponding pathways were shown in the circles and boxes, respectively.
See this image and copyright information in PMC

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